pISceI Dusp6:D2EGFP

LOCUS       pISceI Dusp6:D2EGFP           14535 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pISceI Dusp6:D2EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14535)
  AUTHORS   Molina GA, Watkins SC, Tsang M
  TITLE     Generation of FGF reporter transgenic zebrafish and their utility in
            chemical screens.
  JOURNAL   BMC Dev Biol. 2007 Jun 6;7:62.
  PUBMED    17553162
REFERENCE   2  (bases 1 to 14535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14535
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     11023..11039
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     11024..11040
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      11168..11177
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             11174..12019
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(11219..11240)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11480..11499)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11827..11848
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    12145..12266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12182..12201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12236..12255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(12346..12364)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12386..12402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12386..12402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12399..12421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12410..12426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12434..12464)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12479..12500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12617..12634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12788..13376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12868..12887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13547..14407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14170..14189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14408..14512)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaaa ttaccctgtt atccctataa tacgactcac tatagggcga
      661 attgggtacc gagctcgaat tcatcatacg aattagccac taaatgcaaa agttacgaat
      721 tgctgtgaga ttgtgttgca catttgcatg caaaaacagt acaacgtaaa aagtgtgtgc
      781 tgtgtgtggg tctgtccact gtgtgtgtct ctgtgaactt tgtaatgaca ttatgtgtga
      841 ctcatcaaaa ggcttacatt aaccccacaa caaatacatc aaataatcat tgggaaagtt
      901 tttactgtag tatttctcac aaatattacg tgagatctgc ttccttcatg tctgtcactg
      961 tgctgtttat cttacacagc tgaggcggag atggaggcac actatgactg gcacatggga
     1021 acggtgggtg gggaaaacca gcattaaagg cacagaccac aaaaacagct acaagatgct
     1081 cagagctgat gggtgttttg agctgaaact tcacagacac attctcgaga aaaaaaatct
     1141 taaatcttga aaaaggggta aattaggtgc cctttaagct aaatattttt cctatatata
     1201 tatatatata tataatccaa aaaaaaattt aattcagaac ttgacttctg aatataatcc
     1261 attcaaaagt ctagggtctc gctgattttt taaatttatt tatttattta tttttaagtg
     1321 atttattaaa ttcatcaaaa atatctgtaa aaacatttat aatgttttca aaacattttg
     1381 ataaattttt gtttatcaaa caactcccct accccaacca aaaaaattaa ttatcctgtt
     1441 ttctgaaata ttccttgagc aacaaatcag cgtatgataa ccatttccga agaactgtat
     1501 atatatatat acacacatat acacatacac acacacacac acacacatat tatattcaca
     1561 atgttcacta tgttacataa cagtatacac accttatgag acacacctta gtttgcaatg
     1621 ggaaagtaca gtaagatctt ttttgcgaaa gtgttgggca agtaactagc aataacaact
     1681 ggcagacaca tcttacacca caaagcaatg tgtttataac aacacattag aattaaaaca
     1741 atagtatgga ttgaaatgac tgagacccaa catttcacag actgtgctgc tggtatgtga
     1801 aaattaacta cactgtacag agcgtttcgt tgacttaagt ttaaaaagtg agtaaacttg
     1861 tcgccttaaa atgaataaga acatgaacta tgtgtgtaat tataaaaata aagtcaactt
     1921 agaactttaa cagttctaag ttacaatgtg ttaacttgcc ttttttaagt aaagtcaact
     1981 tgtcgctttt aagccaaata gtttttacgt aaagcaatta atctcttttt acagtgtaga
     2041 taatgcagct atccctaata atatcatcaa tatcaatagc tgtctatata aataacactt
     2101 ttttttgtcc tcttagcagg ataatagatc cgtttgcctc aggccgaaag gagggacaca
     2161 gtaaaataca ctcctgggga tctgctagag atttcaaggc tggtctggtt ttagtggctc
     2221 acaataatgc actctgatca aaacaggaag cagacgcacg agggtctctt gaggaccaca
     2281 gtaaacaaac agccccggtg ggccctcgaa tgccgatccg caaacaggaa gtacaaagaa
     2341 acggttcaga gatcccatga ttagaagtca tcaacaaact attgaggtta ggctttttgc
     2401 tatcgatttg ccttctggta cattattcac gtggtttcat gcatacatat gaattattga
     2461 ttaggatgtg cacatctaaa aagccattag caccccacaa ggcgtttttt tttttttgtc
     2521 gaagcaatgt gaacacaaaa agctctttca atttatcttt ccacatttgt cacttagagc
     2581 tctagtaggt ttaattgcta aaagtatata aaatgtgcaa atacagaagt gtttcattga
     2641 caaccaagca ctttgggttt gtttaaaaac atttaaaagt ctgtaaacgt gtcacaaaac
     2701 catttctgaa gtggcatttc tttatacata tatattttaa gtcagcttta cgctagtgct
     2761 gttaaaagat gaattgcaac taattgcata caaagtatag gtattccttt aagtacatta
     2821 ctctcaccgg ccactttatt aggtacacct tactagtact agttggaccc cctttttgct
     2881 ttcagaactg tcttaatcct tcgtggcatt gattccacaa ggtactggaa atattcctca
     2941 gagattttgg tccacattaa catgatagga tcatgcagtt actgcagatt tgtcggctgc
     3001 acatccatga tgcaaatctc tctttccatc acatcccaaa ggtgctctat tggattgagg
     3061 tctggcgact gtaaaggcca tttgagtaca gtgaactcat tgtcatgttc aagaaaccag
     3121 tctgagataa ttcacacttt atgacatggt gcgttatgct gctggaagta gccatcggaa
     3181 gatgggtaca ctgtagtcat aaaggaatgg gcatggtcag cactcaaact caggtaggct
     3241 gtggtgttga cacgatgctc atttgatact aatggaccaa aaatgtggca agaaaatatc
     3301 ccctacatca ttacacaaac agaagcagcc tgaacagttg atacaaggct ggatggatcc
     3361 atgctttcat gttgttgaca tcaaattttg acctgccata tcaatgtcgc agcagtaatc
     3421 gagactcata agaccaggaa acgtttttcc agtccaattc ctattatcca atttagggag
     3481 tctgtgcgaa ctgtagccag atcagcctat ctggcaccaa aaaaacatgc caaattcagt
     3541 catttatatc acagcagatc gttttgacca tgttcacatg ctttaatgca gtggtcacca
     3601 aacttgttcc tggagggccg gtgtcctgta gattttagct ccaactctaa tcaaacacac
     3661 ctaaacagct aatcaaggtc ttgctaggta tacttgaaac atccaggtag gtgtgttgag
     3721 gctagttgga gctaaaccct gaagggacac cagccctcca ggaccaggat tggtgacccc
     3781 tgctttaatg cattgagttg ctgccatgtt attggctaat tagaaatttg tgctaacaag
     3841 cagttggaca ggtgtacata ataaagtggc cgctgagtgt atatttaata gcattgggtt
     3901 tttacactga attgtgagag gcagagcaat cttggttctg aaaagaaacc tttagtttct
     3961 cattaagcct tggaaattta ttacagtcta aaagtctaaa ggtctaacga gtttttgagt
     4021 tcaatgtatt ttgggatgta atttgcagtt ttcagcatca tcattccagt taacagtgtc
     4081 agatgatcct tcagaaaaca ttctaatgtt caagaaacat ttcttattat tgtcagtgtt
     4141 gaaaacacat tttatattag taaataggta tgtttttccc ttttttagat tttttgtttt
     4201 tgtaaaaata aatagtaaaa ttaaaactta acatttattt aaaatatata tttaatataa
     4261 cttctctctc agctatgatt aatgtaatac ttgcagatga ctcttgctga ttaaaaagat
     4321 ctgattaaaa gactatctct tttaaaataa ataaaagatt gttttaattt aactgattaa
     4381 ttaattgatt gttctgatta ttttccgaat ctaaccattg ggttaaaaag tgtatttgaa
     4441 aattttattc atttacccaa tattgggtta caacaacgta aaatgtttga gtctatgtgt
     4501 agggctacaa caacagaaca ttgaaattta aatagctatt taatagttgc atttaaattt
     4561 ggtgatgaaa ataaaaatac attgtattat gctgttcctt tgtctttttt actgcaatat
     4621 ctgtgtgggt tatcctgtca cttcacttca acccatccac ttttacagca tatacatttg
     4681 tacaaaatat ctaaaacaat cacttttaat ggcttttctc acctctgttt aacctctcga
     4741 ctagctttat ttgcatacat ttttagcttt tttgttgtgt tgtttttttg tgaaagtgca
     4801 gcaacccccc aacagaaatc agcacacaaa gagctccaac agttcgtcaa cagagacata
     4861 aagaggaagt aaacatcacc agagcaggaa gttgcactgt atcatatgtg aagggggggg
     4921 ggggggggcg ttcattaaag aaaagagaaa ccctggtgat tcacaacaca atcagattga
     4981 cctttcatcc caagagctac tctctcaatg atggcagggg gtcaacagag agcgtcccga
     5041 tggagccagt agataaaagc ggagccgcca ttgtctaaag ctctgtagtg gcgcaaactt
     5101 acaaaacatc cagtcccacc caggaactga ggaaaaagaa agaactgtca gctctttcct
     5161 atcaaaccac aatacgtgct aaacagaact agttgacact atagtgcact gtaaagaata
     5221 aataatagta atgttttaat ttaaataata gatttaatta taagcaagtt aatacatttt
     5281 ctaacttttt ttaagttatt ttagctggtt tagttttcag tttagtgtaa tttaagttca
     5341 aatgactcac aacgatttat ttgattatta ttatgtactt tcttattttt cattgaattt
     5401 aatttacctt aaaatttgtt aaagtcttca ttttttacaa aattaaaatt agcaaatgcc
     5461 tcaatttaag tcaaatcaaa taaaatgtac aaggtatttc aacttacatt gcattgaact
     5521 gacttatttt aataagctaa agtaacataa aaaaatgctg tcgtgactta ttttttacta
     5581 taatgtacac tactagtcag tttgcaaagt attttttgta atatcatgat atgttacctt
     5641 tataagcgtt tttaaaaaag tatttcagaa atacttttat tctgcaagga ttcaaatcaa
     5701 agtgactgta aagatgttaa taataatgaa acaattgtgg tttccagaga acagctgaaa
     5761 aatctatctt ttcagcattg atcataatga gaatggatga gaatcaataa ttgttcataa
     5821 cagagcagca aatcaacaaa tgaaaatgtt tttatgtagg atcatctgac acaattctca
     5881 catcacaata ataaaataca ttttaaaata tttcaaaatc caaaaatatc tttttttatc
     5941 tttttacttt tttaatacta ctgttttatt gtaatcttgt agccctgata taatgaaaag
     6001 atttctttca acaacaacaa aaacctatga ccggtagtga attttgattt gaacactgag
     6061 tgcgtgagtg caaatgatct gtatcttgct cttggtacat ttggctaaag aatgtgtgta
     6121 aagatgagaa gtatttgtct gttgcgaggg ctgttttctg ctgtgtttgc ttctgacaca
     6181 gcagatatca tcagcgctgt gataatagca ggaagaagtg agctgttatc agctgctgga
     6241 gccggatgac ctgcatgcgt ctgtttaaaa gacagttgct tgctggtttt atatgacgcg
     6301 ctggactctt atcataatgg tttgaaaaag gactgtttga cagcaaactc gatcatttgt
     6361 tacattaagc taacataatt atctctaaaa aattctcaaa atttgtttac agaaatcaat
     6421 tatatctgtg aattgtatta acatataaca aatcagtcat tttgagttca tttatcaaac
     6481 atttagatgt atgatcagat cttatatttt ttctttgtgg caaccatttt tttatgtgct
     6541 attaataata ataataataa taataataat aataaatcag tcacatattt gatgccattt
     6601 atattatttc taagaaaaat ttcaattatt caactttgat gcatttccca cttcaattta
     6661 tgtctaataa attattatta tttctgttta gcaatagtcc tatttatttt aaagccctta
     6721 ttcacttctt gcatttaatg tttataatcc catatgagcc attttatgtt tgtgtgtagt
     6781 aggaactcta ctgtttatta ttcattcatt ttcctttggc ttagtctctt tattaatttg
     6841 gggtagctac agcagaatga tccaccaact tatccagcat atgtttttac actataaaat
     6901 tcaatgtacc aataatgcat gtctttggac tctgaggaaa accagagcac ccggatgaaa
     6961 cccacgtcaa catggggaga acatgcaaac tccacagaga aatgccaact ggcctagtcg
     7021 gcactcaaac cagtgatctt ctcgctgtga ggtcacagtg ctaaccactg aaccattgtg
     7081 tcgacctact ttttaatata tattttttaa tacttatcat atatttaatt tgaagatcaa
     7141 acatctaaaa tagaaggaat gtaaatagtt ttagtaagta tgttagtatt aagctataca
     7201 ttttatttat attcatcctt aaagggatag ttcaccccaa atgaaagatt tcctctctct
     7261 caagttactg tagtttcttt ttattgaaca caaaagaaaa tattttgaag aaagctgaag
     7321 actacttaca tccatagtag aaaaaacaaa tactatgaaa gtcagtggtt acaggttttc
     7381 agcttttttc caggtttcct tttgtggtat acagaaaaat gaaacttaaa ccaatttaga
     7441 acaagtgaag ggtgagtaaa cggtgacaga attttaattt catatatgaa ctatcccttt
     7501 aaacaacagc atctgtgttt cataagatga catattctag aaaaagacaa aagctcatcc
     7561 acatgcactg tcttcaggca ccaaagcagc caaattggat tacaatgaga atacaattca
     7621 tcctttccag aaagatctcc acaagccaca ggatagaggc cattatctgg gcactgtgtc
     7681 ctgtttcata tgttaagcgg ggtgaaactg aacacacctt tggcccatgt gaccatcctg
     7741 catgtgaatg atgagagaag gccttgatag acaattaaag agcaattcac tgccaaatga
     7801 cacaggagac aggatgtctg acagcatgtt aatcaggtca cactaatctg gcctactgca
     7861 ttcattctgg ccaaacgcat attccagtct aaaaaaataa agaaggcatc tttattggca
     7921 tcaatggttc catgaagaac ctttaataac cattgaatct tttcagcaca caaaaggttc
     7981 tttaaacctt ttttcccact gcgggtacac agatctttga ggagctaagg gctcattgag
     8041 gaaccaaaac atttactgtg gcattacagt gacaggcccg tagacagggg ggttcgggtg
     8101 gttcgaaaga cacacccctt attgacaaag gtccagaact tgtcccatac ataagctcat
     8161 ttgttttatt ttgactgcta tgccatcata aatagtaaaa ttaatccatt gaaaaaggct
     8221 ttaagaccaa gcaaaatcct atattaaaat gaatttgaaa gctaatttgg ctatttttgt
     8281 tatccatgaa ttttcaatca aatcaaatgt actttttata caatagaggc tagaaaaatt
     8341 gtgaagctct ttattattat tattattatt attatacttt atttttttat gatttaaata
     8401 gacaaattct gactgaggac agttaaatgt ggccagttta taggctacgg ctacaatagg
     8461 ctacagtttt taattattaa tttattcttc tttttttttt aatctttagg aaatattttg
     8521 atacattaaa tgtgtcatta tgatgacaat ctaacaagct aatcctgcta aaatagtgat
     8581 taatatgtca ctaaaataca gtatttaaat ctcatacatt gtaaatgggg tgaaaaactg
     8641 caaaaaggtc cacattttga gaaaaagaac caccccttgt accagactgg ctacaggcct
     8701 gagtgaaaaa cacattttgg attaaaaaat tgtgtactct gttattattt taacataata
     8761 aagtgtttgc acaggcaata atttactcac tctgatgtat ttttttcaca agatggaaga
     8821 tttaattttt ggactcagtt gatctttgtt tagtatagag cagctttgag cttaattcac
     8881 acacaggatt gtcctgtgta cacatgccac atttttcatg tgctctggtg ctaaactata
     8941 gggtttggca aaaaaaaaaa aacaaaatat aacataaaat gtcacaaaaa ttcaaagtat
     9001 gactatagaa taatggttgg tcttctgcta ctgcgcagaa gttcacttag acagtgtgtc
     9061 agtaaaaaag taaaaaaaaa attccccatt ttcagttaag cacgaactaa ctgtttaaat
     9121 tccatttata gtctatctac ataataacat gtccctaagc aatgttttct cgcctacgta
     9181 atgcaaattg agcaagctga atagcatttg gccacttgtt ttgagcattt ctaaacgcca
     9241 gagaccaggc gacctgacct ttttaacgaa cactagttta tcagtatgca attcaaatga
     9301 accctgttct actgcaactg atatagcaat tattaaaact gtgtggtaac gacacgagaa
     9361 aatattgtca gtgacatctg ctttaatgca tttgtgcgaa tccggtgcag ctgttgaggg
     9421 atttgtgaat gatttttttt taagctggca cacacaagat gacgttaggt tcattggagg
     9481 aaatgaccga agaggaggcg aggaaacact ggcaattcgc atctgctgtt aaactatttt
     9541 gatggctcca tttatgtgtt tattaggcga tcatttagcg cgcgcttcca cattcattga
     9601 atttggattg agcaatcgat catcaaggta ataatctgat gagagcatgt gcgctttatt
     9661 gcgattacgt gatctaattg tactttcata tgttcgagtt acaagagata ttagcccaca
     9721 gtaaaatggg ggctaatcta aattagggat ttttgcagca atacttcgga ttttacttag
     9781 catatttttc agaagtaatt taataacaca tacaaatttc atggatatta tggttataca
     9841 gaagcttttt gaaaataaaa tgcaatgatc tcttttttac gtgtaatgta ttgggatatg
     9901 cctattgttg ttattattta caaattgtaa tttggcaaat attttcttga tttatttgct
     9961 cttcagtgtt tggactttca gcagtgaaaa ccacactgaa ctgagctaaa ctgaacttca
    10021 actctgaaaa ctgaactgac agtttcaatt taacagaact tctctgttaa gctgctttga
    10081 cactagtgtg cattgtgaaa tctttataaa ataaagatta ctttaatttt gatatgttgt
    10141 gtaaaactga aatatgtatt ttatttgtgc atacgacacc aaaaaggact aatttgagac
    10201 agtatatatc cgcgttacac tgttttacag tacgtcagct gcgtaccatg ctccgcaccg
    10261 ccgctggacg aaacagaaac acgactaaac gagttcaagt gtgtaacctg agcacgaggt
    10321 atatgaaccc ctcaccaaac aacactcgca ctcctccttc cttcccgtga agcgcctctc
    10381 tactgtttgt ttgcactccg ctgatccgga gcggaaattc ctttccgttt ttgtgaatga
    10441 caaacttatt aacaattcat caacacagtc tgttccatcc ggccccgttt ccaggcgacg
    10501 atcgctcggc gcctcctgat tggctggccg acataatgta tccattgaaa cgcctcgcgc
    10561 ttgtatccat tcactagctc acagtgacag agagcgcatc tcggacttgt ggagcggagg
    10621 acgcagtgtt gcattctcca ggataaacat cgaaaggaaa taacaatacg aagcattttg
    10681 cttttgcaat cgacattcaa ctggatatct agagcgcttc gtgcgaaaat cgacgacgag
    10741 agaagagagg ttatataata ggaagacaac gtcagtggct cgcgcactca caggctagtg
    10801 tgagtcattg ataaatgcac acagttgcat gaatggggtg gaagtagatc gaggctgcgc
    10861 ttgactctcg aaacaaacaa aaaacgagga ttttaatcat ctcgacggcg cacaagagac
    10921 tttggcgtgt aaaaagctca atgctccagg cctgagacgt ctacggcctc ttgcagcgcg
    10981 tcttctccgc agttttaagg tctcacggta ccgggccccc cctcgaggtc gacggtatcg
    11041 ataagcttga tatcgaattc ctgcagcccg ggggatccgt ttaaacacgg ccattacggc
    11101 caaactcgct cgcccgggcc gaaccagtgg ctgcagggcc gcctcggcca aaggcttaat
    11161 taaggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
    11221 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
    11281 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
    11341 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
    11401 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
    11461 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
    11521 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
    11581 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
    11641 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
    11701 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
    11761 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
    11821 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
    11881 gctgtacaag aagcttagcc atggcttccc gccggaggtg gaggagcagg atgatggcac
    11941 gctgcccatg tcttgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgcttc
    12001 tgctaggatc aatgtgtaga tgcgcggccg cgactctaga tcataatcag ccataccaca
    12061 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    12121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    12181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    12241 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata gatctttact
    12301 agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
    12361 aattaccctg ttatccctaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    12421 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    12481 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    12541 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    12601 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    12661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    12721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    12781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    12841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    12901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    12961 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    13021 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    13081 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    13141 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    13201 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    13261 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    13321 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    13381 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    13441 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    13501 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    13561 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    13621 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    13681 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    13741 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    13801 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    13861 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    13921 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    13981 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    14041 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    14101 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    14161 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    14221 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    14281 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    14341 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    14401 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    14461 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    14521 cccgaaaagt gccac
//